Mammalian sex hormones influence germination velocity and enzyme activities in germinating maize seeds
Effects of mammalian sex hormones (progesterone and (3-estradiol) on growth parameters (percentage of germination, root and shoot length) and activity of selected enzymes (a-amylase, superoxide dismutase, peroxidase, cata-lase and polyphenoloxidase) in germinating maize were studied. Maize seeds were germinated on Petri dishes in the solutions of steroids (concentrations: 10 -4 10-6, 10-9, 10-12, 10 -15M). Steroids in all tested concentrations significantly increased root and coleoptyle lengths as well as percentage of germination of maize in comparison to control. The highest germination ratio, root and coleoptyle lengths were recorded at 10-9M and 10-12M of both progesterone and ?-estradiol. Likewise, progesterone and ?-estradiol in all tested concentrations stimulated the activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), polyphenol oxidase (PPO) and aamylase. The highest activities of these enzymes were determined in endosperm of seeds under the influence of progesterone and Pestradiol at concentrations with the highest root and coleoptyle lengths as well as percentage of germination.